Tetrodotoxin and its analogues profile in nemertean species from the sea of Japan

Abstract For the first time search for tetrodotoxin (TTX) and its analogues in the extracts of nemerteans using HPLC-MS/MS was performed. TTX analogues were detected in two nemertean species in addition to TTX: 7 analogues were detected in the extract of Cephalothrix simula , 3 analogues - in the extract 11-norTTX of Kulikovia manchenkoi . Presence of 5-deoxyTTX, 11-deoxyTTX, 5,6,11-trideoxyTTX and −6(R)-ol in nemerteans was shown for the first time.