The anthocyanin profiles from Sambucus williamsii hance and analysis of its anti-Transmissible gastroenteritis virus (TGEV) activity.

Transmissible gastroenteritis virus(TGEV) can make the pig infectious gastroenteritis disease, and lead to death of large numbers of the pigs(Sus scrofa). Our study investigated that the inhibitory activities of anthocyanins from Sambucus williamsii Hance to TGEV in vitro. Anthocyanins structure of S. williamsii Hance was identified by LC- MS. Prevention and treatment roles of anthocyanins in the TGEV- infected different phase were studied, 3-(4,5- dimethylthiazol- 2- yl)- 2,5- diphenyltetrazolium bromide(MTT) and inverted microscope assay were used to observe the swine testicular(ST) cell survival rate and cell morphological changes. The results showed that S. williamsii Hance contained six major anthocyanins, and one of the main component was cyanidin- 3- glucoside. ST cell was well- grown without the cytotoxic response when treated with anthocyanins lower than 50 μg/mL. Anthocyanins could restrain the proliferation of TGEV under 10 μg/ mL, and the inhibition effect showed as dose- dependent manner in the safe concentration. In cell culture condition, the prevention was better than treatment effects about anthocyanins anti-TGEV. In short, this paper identified that there were at least six kinds of anthocyanins component of Sambucus williamsii Hance, and the best activity of anthocyanins against TGEV was about 50 μg/mL, and this will provide theoretical basis for the development of the new plant source of antivirals medicine.