The influence of ischemia time in injury of deep peribiliary glands of the bile ducts graft: a prospective study

Abstract The deep peribiliary glands (DPBG) are a niche of progenitor cells in the wall of the biliary duct (BD) and are the second line of multiplication when severe lesion of the epithelium occurs. Previous studies have identified DPBG injury as a cause of post liver transplant (LT) biliary stenosis; this complication is a major cause of post LT morbidity. The incidence of biliary stenosis in our center is high (38.1%). This study evaluates the lesion of DPBG in response to ischemia. Graft BD was collected in adult LT between 08/2016 and 07/2017, from donation after brain death (DBD). Samples of 45 grafts were collected at two moments: BD1-during graft preparation and BD2-before biliary anastomosis. Histological analysis of the samples was performed and then classified according to degree of lesion (0, ≤50% and> 50%). A comparison was made between the degree of lesion and graft ischemia, graft histology, donor and procurement variables. The DPBG lesion was more frequent in BD2 (20.9% vs 7%, p = 0.079). BD2 lesions with DPBG lesions had higher medians and means at all times of ischemia. The difference was greater in the warm ischemia time (WIT) (0:43.3’±12.53 vs ≤50%:52.4’±14.38, p =0.068). The group of BD1 with DPBG lesion presented superior median cold ischemia time (CIT). In the analysis of the remaining variables there were also no statistically significant differences. We concluded that during the period of CIT there is already lesion of the DPBG, which increases after reperfusion of the graft, in greater association with longer WIT.