MICROFLUIDIC SYSTEM FOR MULTICHANNEL OPTICAL MEASUREMENT OF SHEAR-INDUCED PLATELET THROMBOSIS IN UNFRACTIONATED BLOOD

We report on development of a microfluidic device and optical system for measurement of occlusive platelet thrombosis at initial shear rates ranging from physiological to pathological conditions from (500 ‐ 13000 s -1 ) within four stenotic channels in unfractionated blood. Real-time, label-free measurement of platelet thrombosis was performed by measuring optical radiation transmitted transversely through flow channels. Occlusive thrombosis occurred at shear rates above 4000 s -1 and measurements showed average differences in of 8.4% and 3.97% compared with traditional flow-rate and white-light microscopy methods, respectively.