Improved CTAB Method for Total RNA Extraction of Mature Feicheng Peach Fruit

In order to extract pure and integrate RNA from Feicheng peach fruit,an improved CTAB method was developed,and the integrity,productivity and purity of the extracted RNA were evaluated in this paper.The results showed that the RNA extracted by the improved CTAB method had good integrity and the RNA bands were clear without degradation.The ratio of the brightness of 28S rRNA to that of 18S rRNA was approximately 2∶1;the A260/A280 was 1.89,and the yield of total RNA was 356.21 μg/g.After RT-PCR,a specific band was obtained,indicating that the extracted RNA could be used for the following molecular biology operations.