Simultaneous determination of eight biogenic amines in the traditional Chinese condiment Pixian Douban using UHPLC-MS/MS.

Abstract A UHPLC–MS/MS method was developed to simultaneously determine eight biogenic amines (BAs) in Pixian Douban. Under optimal conditions, the linear ranges of determination were 5–1000 μg/L (that of spermine was 8–1000 μg/L). Correlation coefficients ranged from 0.9955 to 0.9987. The limits of detection were 0.11–5.5 μg/L. The matrix effect and analytical performance of the present method were evaluated, and the eight BAs were analyzed by this method in 19 samples, indicating the potential pollution of BAs in chili oil Pixian Douban.