Effect of immune sera against polyvalent protective antigen of Plasmodium falciparum on the development of asexual stage of Plasmodium falciparum FCC1/HN isolate in vitro.

The effect of immune sera against purified and unpurified recombinant antigens of Plasmodium falciparum on the development of asexual stage of P. falciparum FCC1/HN isolate was observed in vitro. The results demonstrated that the growth and development of P. falciparum were strongly inhibited by the sera against purified and unpurified recombinant antigens. The inhibitory efficacy was related with the concentration and incubation time of the immune sera. When the sera diluted at 1∶40, 1∶80 and 1∶160, the inhibitory rates of sera from purified and unpurified recombinant antigen immunization at 72 hours were 67.91%,43.28%, 24.63% and 58.21%, 52.24 %,38.06% respectively. In addition, the development and morphology of parasites were abnormal when incubated with the immune sera. It suggested that the polyvalent antigen PfCMR can induce protective immunity against asexual stage development of P. falciparum FCC1/HN in vitro.