Genotoxicity effects of Phanerochaete chrysosporium against harmful algal bloom species by micronucleus test and comet assay

Abstract Algal blooms and toxins have become serious ecological problems. White-rot fungi have been demonstrated to be a feasible means of control, but the genotoxicity mechanisms involved have not been reported. In this study, Cryptomonas obovata FACHB-1301, Oscillatoria sp. FACHB-1083, and Scenedesmus quadricauda FACHB-507 were co-cultured with Phanerochaete chrysosporium under optimal conditions of 250 mg-l at 25 °C with DO 7.0 mg-l for 1, 3, 5 and 7 d. Compared to the control groups, the values for tadpoles exposed to algae treated with Phanerochaete chrysosporium were only increased from 1.95 ± 0.09, 2.78 ± 0.08 and 2.37 ± 0.13 to 2.45 ± 0.07, 3.56 ± 0.08 and 2.54 ± 0.10, and the frequency of nuclear anomalies reached 6.45 ± 0.06, 11.14 ± 0.05 and 7.85 ± 0.10 to 7.68 ± 0.08, 13.12 ± 0.06 and 8.57 ± 0.12 in the experimental groups after 7 d. What's more, the tail lengths were only increased to 36.77 ± 0.54, 41.58 ± 0.78 and 35.38 ± 0.66, and the comet length reached 55.67 ± 0.68, 68.56 ± 0.85 and 51.43 ± 0.82. The results demonstrated that Phanerochaete chrysosporium effectively decreased genotoxicity effects in Fejervarya multistriat tadpoles. These results could provide new ideas for inhibiting water blooms, and lay a theoretical foundation for promoting the deepening of water eutrophication.