EFECTO DEL PRE-TRATAMIENTO CON UN EXTRACTO DE CAESALPINIA SPINOSA ANTE LESIONES EN MUCOSA GÁSTRICA INDUCIDAS CON ÁCIDO ACETIL SALICÍLICO EN RATAS HOLTZMAN

Objective: to determine the effect of the ethanolic extract of Caesalpinia spinosa’s pod against acetylsalicylic acid-induced gastric mucosal injuries in male Holtzman rats. Methods: Incomplete experimental study. It was done at the Institute of Pathology of the National University of San Marcos, Lima, Peru. The participants were 34 male Holtzman rats. 5 groups were formed which received a 6-day course of treatment with either table water (negative control and ulcer control groups) or ethanolic extract of Caesalpinia spinosa’s pod in doses of 100, 200 y 300mg/kg (treatment groups) given by orogastric gavage. After 1h from the last administration, gastric mucosal injuries were induced in the last 4 groups with aspirin 400mg/kg given by orogastric gavage. Main outcome measures: macroscopic and microscopic qualitative assessment of the gastric mucosal injuries’ severity and macroscopic quantitative assessment by means of measuring of the injured area. Quantitative outcomes were compared by means of Kruskal-Wallis (p<0.05) and Mann-Whitney (p<0.05) tests. Results: significant differences were found in the percentage of injured area between all treatment groups and the negative control group but not between the negative control group and the ulcer control group nor between the treatment groups and the ulcer control group. Conclusion: ethanolic extract of Caesalpinia spinosa’s pod does not have a protective effect against acetylsalicylic acid-induced gastric mucosal injuries in male Holtzman rats.