Development of metabolically engineered Corynebacterium glutamicum for enhanced production of cadaverine and its use for the synthesis of bio-polyamide 510

Lysine decarboxylases (LDCs) from Escherichia coli, Lactobacillus saerimneri, Streptomyces coelicolor, Selemonas ruminantium, Hafnia alvei, and Vibrio vulnoficus were examined for their ability to enhance the fermentative production of cadaverine in Corynebacterium glutamcium. Among these LDCs, the plasmid-based expression of H. alvei LDC gene (ldcCHa) under strong promoters (PH30, PH36) produced high concentrations of cadaverine (11.4-11.5 g/L), which is similar to 12.5 g/L of cadaverine produced by plasmid-based expression of E. coli LDC gene (ldcCEc) by PH30 promoter in our previous report. The production of cadaverine in batch (30.8 g/L) and fed-batch (93.7 g/L) fermentation cultures using recombinant strain C. glutamicum H30HaLDC expressing plasmid-borne ldcCHa under control of the PH30 promoter was 18% and 14% higher than that obtained using C. glutamicum P-H30 expressing plasmid-borne ldcCEc under control of the PH30 promoter, in which production was only 26 g/L and 82.2 g/L in batch and fed-batch ...