Analysis of Mycoplasma agalactiae and mycoplasma bovis antigens by polyacrylamide gel electrophoresis (page)

Polyacrylamide gel electrophoresis of two Indian isolates of M.agalactiae (RPNS 200 and RPNS 216) and one isolate of M. bovis (NC317) was carried out using two types of antigens viz. whole cell antigens (WCA) and sonicated supernatant antigen (SSA) under non-reducing and non-denaturating conditions. The results revealed 5 to 9 polypeptides in the region of 160.32 to 19.95 kDa. Protein profiles of WCA of M.agalactiae and M. bovis were almost similar and revealed 5 polypeptides namely, 160.32, 107.15, 97.70, 44.66 and 28.84 kDa. The SSA of both the species has the difference in polypeptides, as the bands of 70.70, 33.88 and 20.89kDa were present only in the isolates of M. agalactiae, while the polypeptides of 20.50 and 19.95 were present in M. bovis. These polypeptides can be used for species identification and differentiation.