Analysis of antioxidant enzymes combined with the detection of MCL-1 and Bax expressions in acute childhood leukemias

17522 Background: Occurrence and biological characteristics of tumors are related not only to over-proliferation of carcinoma cells but also to decrease of apoptosis. Investigation of apoptosis helps to disclose the biological characteristics of tumors, and seeks new methods of diagnosis and treatment for tumors. In this study, we analyzed the level of some antioxidant enzymes in various leukemic patients including SOD. Methods: To investigate the involvement of apoptosis regulating proteins in acute leukemias, immunocytochemical examination of Mcl-1 and Bax proteins expression was investigated in two groups of patients, one with acute lymphoblastic leukemia (ALL) and the other with acute myeloid leukemia(AML). Moreover, the free radicals utilizing enzymes were sought including superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), and catalase (CAT), in addition to malonaldhyde (MDH), nitric oxide (NO), and glutathione content (GSH). Results: The obtained results showed a significant difference of ...