MP1-19 A NOVEL LIVE CELL MICROFLUIDIC DIAGNOSTIC USING PHENOTYPIC BIOMARKERS WITH OBJECTIVE ALGORITHMIC ANALYSIS FOR PROSTATE CANCER RISK STRATIFICATION.

PTEN IHC was 62% sensitive for detection of hemizygous PTEN deletion by FISH, with homogeneous or heterogeneous PTEN protein loss in 30/48 tumors with hemizygous deletion. Other inactivating mutations of PTEN (not detectable by FISH) may lead to PTEN protein loss in tumors with normal or hemizygous PTEN copy number. CONCLUSIONS: Our validated and automated PTEN immunostaining protocol is a simple and relatively inexpensive test that is 91% specific and 98% sensitive for detection of homozygous PTEN gene deletions in a large multi-center study of prostate cancer.