Diterpene compound variediene synthesizing gene Au13192 and application thereof

The invention discloses a diterpene compound variediene synthesizing gene Au13192 and application thereof, and belongs to the field of gene engineering. The diterpene compound synthesizing gene is derived from aspergillus 094102, a gene and a cDNA sequence of the diterpene compound are shown in SEQ ID NO.1 and 2, and a sequence of encoded Au 13192 protein is shown in SEQ ID NO.3. Au13192 protein has functions of catalyzing substrate chain length to extend and structure cyclizing, and the protein can catalyze DMAPP, GPP and FPP to react with IPP and generate variediene. The invention finds that an Au1392 gene has remarkable influence on yield of ophiobolin type compound in the aspergillus 094102, and the Au1392 gene can be used for preparing an Oph compound adjunctively. The invention provides a new resource for biosynthesis of the Oph compound, and provides possibility for improving biological yield of the compound.