Method for preparing rabies vaccine
2012
The invention discloses a method for preparing a rabies vaccine, and belongs to the technical field of organisms. The method comprises the following steps of: a, cell inoculation; b, cultivation of cells for seeding; c, virus vaccination, d, virus cultivating and harvesting; and e, vaccine preparation. By adopting the method, the virus is rejuvenated; the reproductive capacity of the virus is enhanced; the multiplication titer and the harvesting yield of the virus are improved by adopting a torrent perfusion-type bioreactor culture system; the entire production process is not involved with other problems of biosafety and public health; and the method is suitable for mass production.
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