Fibroblast growth factor-10 prevents H2O2-induced cell cycle arrest by regulation of G1 cyclins and cyclin dependent kinases

Abstract We studied the effects of fibroblast growth factor (FGF-10) on H 2 O 2 -induced alveolar epithelial cell (AEC) G1 arrest and the role of G1 cyclins. FGF-10 prevented H 2 O 2 -induced AEC G1 arrest. FGF-10 induced 2–4-fold increase in cyclin E, cyclin A and CDKs (2, 4) alone and in AEC treated with H 2 O 2 . H 2 O 2 downregulated cyclin D1; FGF-10 blocked these effects. FGF-10 prevented H 2 O 2 -induced upregulation of CDK inhibitor, p21. SiRNAp21 blocked H 2 O 2 -induced downregulation of cyclins, CDKs and AEC G1 arrest. Accordingly, we provide first evidence that FGF-10 regulates G1 cyclins and CDKs, and prevents H 2 O 2 -induced AEC G1 arrest.