Gene Cloning of Porcine Fc Receptor Gamma Subunit and Its Prokaryotic Expression

mRNA encoding porcine FcR gamma subunit obtained from PAMs of 90-day piglets was transcribed into cDNA through RT-PCR.Recombinant plasmid pET-FcRγ was constructed successfully by inserting the cDNA sequence into pET-32a,and expressed as soluble protein with a molecular weight of about 29 kDa.Following purification,recombinant protein FcRγ-His was used to immune mouse,and polyclonal antibody against it was obtained.Western-blotting indicated that such polyclonal antibody could react specifically with FcRγ-His.Indirect ELISA shows that the titer of the polyclonal antibody is 1 ∶ 8000,revealing the good immunogenicity of FcRγ-His.All these results provide the foundation for further studying the structure and function of FcR gamma subunit.