The tattoos of the Tyrolean Iceman: a light microscopical, ultrastructural and element analytical study

The Tyrolean iceman, a 5300-year-old mummy, presents tattoos on different parts of his body. Skin samples of several line tattoos and a cross tattoo were investigated by optical microscopy and various electron microscopy techniques (TEM, EFETEM, EELS, EDXS, electron diffraction). The epidermis of the investigated skin areas was mostly lacking. The tattooing particles are distributed in the connective tissue and could be identified as soot. In some areas between the soot particles, different silicate crystals are present such as almandine and quartz, along with some not exactly definable crystals. The crystals possibly derived from stones of the fireplace from which the soot was taken for tattooing. Additionally, in the medial part of the right leg, putative ash particles were seen in the tattooed area. Furthermore, in non-tattooed skin areas, a vivianite crystal and small grains of CaPO4 and nearby Fe, P and O could be detected, these having formed most probably after the death of the Tyrolean Iceman during his long period in the mountains. On the surface of two leg specimens, a small layer with brown granules was visible by means of optical microscopy, and these were identified as melanin granules in the stratum basale of the epidermis.