Abstract 689: Methylation of miRNA genes is a useful biomarker for detection of bladder cancer.

Background and aim: Dysregulation of microRNAs (miRNAs) has been implicated in bladder cancer (BCa), though the mechanism is not fully understood. We aimed to explore the involvement of epigenetic alteration of miRNA expression in bladder cancer. Materials and methods: Two BCa cell lines (T24 and UM-UC-3) were treated with 5-aza-2’deoxycytidine (5-aza-dC) and 4-phenylbutyric acid (PBA), after which their miRNA expression profiles were analyzed using a TaqMan Array. Bisulfite pyrosequencing was used to assess miRNA gene methylation in 5 cancer cell lines, 83 primary tumors, and 120 preoperative and 47 postoperative urine samples. Receiver operating characteristic curve analysis was used to assess the diagnostic performance of the miRNA gene panel. Results: Of 664 miRNAs examined, 146 were upregulated by 5-aza-dC plus PBA. CpG islands were identified in the proximal upstream of 23 miRNA genes, and 12 of those were hypermethylated in cell lines. Among them, miR-137, -124-2, -124-3 and -9-3 were frequently and tumor-specifically methylated in primary cancers (miR-137, 68.7%; miR-124-2, 50.6%; miR-124-3, 65.1%; miR-9-3, 45.8%). Methylation of the same four miRNAs in urine specimens enabled BCa detection with 81% sensitivity and 89% specificity; the area under the ROC curve was 0.916. Ectopic expression of silenced miRNAs in BCa cells suppressed growth and cell invasion. Conclusions: Our results indicate that epigenetic silencing of miRNA genes may be involved in the development of BCa and that methylation of miRNA genes could be a useful biomarker for cancer detection. Citation Format: Hiromu Suzuki, Takashi Shimizu, Masanori Nojima, Eiichiro Yamamoto, Reo Maruyama. Methylation of miRNA genes is a useful biomarker for detection of bladder cancer. [abstract]. In: Proceedings of the 104th Annual Meeting of the American Association for Cancer Research; 2013 Apr 6-10; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2013;73(8 Suppl):Abstract nr 689. doi:10.1158/1538-7445.AM2013-689