In vivo and in vitro characterization of probiotic organisms for their microbial adhesion property isolated from Coconut toddy

Abstract In current scenario it is a big challenge for keeping good health. The adulterate food we get in the market makes the researcher to think for safeguarding their digestive system. To solve the problem probiotics which are the live bacteria and yeasts helps in our good health, especially our digestive system. To keep our gut healthy the present study has focused on two probiotic organisms namely Lactobacillus fermentum ( KT183369 ) and Bacillus subtilis sub sp. Inaquasporium ( KR816099 ) were isolated from coconut toddy and they were optimized for their probiotic nature. Further, the probiotic bacteria were analysed and have been found to show good adhesive property towards the host cell. During this study, the adhesive properties of probiotic bacteria could be identified by using ornamental fish black moley ( Poeciliasphenops ) and also by Microbial Adhesion To Hydrocarbon assay (MATH assay) using toluene as solvent. The probiotic bacterium showed good adhesive properties in both in vivo and in vitro studies using aquatic fish and its pathogen, Vibrio paraheamolyticus respectively. Additionally, during this study, the fishes were infected along with the probiotic feed to check their mortality rate and the hydrophobicity percentage of the probiotic organisms was also calculated using the assay method during.