Innate immune adherence activity to tumor cells and phenotype properties of Vincristine-loaded intact human erythrocytes

Objective:To study the effect of VCR-loaded process on innate immune adherence activity to tumor cells of erythrocytes and explore its molecule mechanism preliminary.Method:VCR was loaded in human erythrocytes using the modified hypotonic preswelling technique.We used the erythrocytes after undergoing washing,swelling and drug-loading treatment from healthy donors and tumor patients,and compared their innate immune adherence activity to tumor cells through measuring the rate of rosette formation and expression of CD35,CD44,CD55,CD59,and CCR4 by flow cytometry.Result:We observed that the innate immune adherence activity of erythrocytes from tumor patients after hypotonic swelling treatment significantly increased as compared with other groups(P(0.05)) with the rate of rosette formation up to((39.20)±(18.14))%.The innate immune adherence activity of erythrocytes from healthy donors slightly increased after drug-loading.The rates of rosette formation before and after drug-loading were((48.30)±(13.52))% and((51.20)±(11.24))% respectively,both are significant higher than those from tumor patients(P(0.05)).FACS analysis demonstrated that expression of CD35,CD44,CD55,and CD59 on erythrocytes was down-regulated after washing,swelling or drug-loading treatment,but CCR4 was up-regulated.Conclusion:VCR-loaded treatment did not affect the innate immune adherence activity of erythrocytes to tumor cells significantly.