북방전복 (Haliotis discus hannai) 정자의 동결보존에 대한 희석액과 동해방지제의 영향

The purpose of this study was to examine various cryoprotective agents (CPAs) and diluents on post-thaw motility and fertility of Haliotis discus hannai sperm based on movable sperm ratio (MSR), sperm activity index (SAI), fertilization and hatching rate. To evaluate the optimal CPA for cryopreservation of H. discus hannai sperm, the sperm was diluted in three extenders (sea water filtered, 1 M glucose, 1 M, sucrose) containing one of three CPAs(dimethty sulfoxide [DMSO], glycerol [G], ethylene glycol [EG]) at the concentration of 1, 2.5, 5, 10, 20%, and then frozen in programmable freezer. Sperm diluted with sea water containing 2.5% DMSO had the best post-thaw MSR (40.0 ± 2.9%), SAI (3.3 ± 0.1%), fertilization rate (50.8 ± 15.0%) and hatching rate (76.5 ± 4.5%).