Role of Pim1 Gene Overexpression in Pathogenesis of Acute Myeloid Leukemia

题目: Pim1基因过表达在急性髓系白血病发病机制中的作用研究. 目的: 研究Pim1上调对急性髓系白血病（acute myeloid leukemia，AML）细胞系U937增殖、凋亡、趋化作用和血管生成的影响，探讨其可能的作用机制，了解Pim1基因在AML患者原代细胞中的表达情况. RESULTS: The lentivirus-infected AML cell line with Pim1 overex-pression and the control virusinfected AML cell line were established successfully. The Pim1 overexpression could enhance the proliferation and inhibit the cell apoptosis, moreover accompnied with the increasing expression of cyclin D1, phosphorylated BAD (pBAD) and pCXCR4. After SDF-1 α stimuli, Pim1 overexpression induced AML cell chemotaxis accompanied with p-CXCR4 expression and calcium influx increment. Pim1 overexpression has no effect on angiogenesis. Pim1 mRNA expression was significantly higher in AML patients than the healthy people. CONCLUSION: Pim1 plays an important role in the pathogenesis of AML, which not only promotes AML cell proliferation and inhibition of apoptosis, but also enhances the chemotactic ability of leukemia cells, which closely relates with Pim1 phosphorylation of CXCR4 and the increase of intracellular calcium ion influx signals. 结论: Pim1在AML发病机制中起到重要作用，其不仅促进AML细胞增殖和抑制凋亡，还能提高白血病细胞的趋化能力，这与Pim1磷酸化CXCR4和增加细胞内钙离子的内流信号有密切关系.