BIOFILM PRODUCTION BY RESISTANT NON-FERMENTING GRAM NEGATIVE BACILLI FROM INTENSIVE CARE UNITS

OBJECTIVE: to verify the presence, resistance profile, as well as bacterial biofilm production of non-fermenting Gram-negative Bacillus isolates from tracheal secretion, blood culture and surfaces of an Intensive Care Unit. METHODS: MacConkey Agar, TSI and oxidase test were used for bacterial identification. Antimicrobial resistance occurred by the Kirby-Bauer disk diffusion method and the presence of bacterial biofilm was verified by Quantitative Microtiter Dish Biofilm Formation assay. RESULTS : of 30 isolates of negative Gram, seven were NFGNB. Four of the genus Acinetobacter sp ., two of the genus Pseudomonas sp ., and one of the genus Burkholderia sp . was identified. The results showed moderately and strongly biofilm producing isolates. CONCLUSION: the bacteria found showed resistance the antibiotics tested associated with biofilm production biofilm. Identification these pathogens can greatly contribute to more effective hospital antibiotic therapy. Keywords: Biofilm; Microbial Drug Resistance; Intensive Care Unit, Bacteria.