Effect of chemical modification of isoenzymes A and B from C. rugosa on their activity and stability

Lipases A and B from C.rugosa have been chemically modified with octanoyl chloride or polyethylene glycol (molecular weight: 750 or 5000). Although the hydrolytic activity was diminished, modification gave rise in some cases to preparations with increased synthetic activity in hexane. Furthermore, PEG5000-lipases A and B were significantly more stable (up to 20-fold) at alkaline pH values than the native enzymes.