The Diffusion Chamber Technique as an in Vivo Assay in Mice for the Effectiveness of Antitumor Agents

The diffusion chamber (DC) technique has been applied to the culture of L1210 cells in view of determining their sensitivity to chemotherapic agents. The surviving fraction of L1210 cells cultivated in DC's 4 d after i.v. injection of the host mice with 10 mg/kg of daunorubicin or daunorubicin-DNA was 40% and 9%, respectively. The results suggest that the DC technique could be a useful in vivo predictive test for cancer chemotherapy.