Agrobacterium-mediated Transient Expression of Foreign Gene in Arabidopsis thaliana

Viral vector -mediated inoculation is a normal method of transient expression of foreign gene in the plants. In this study, we transiently expressed the foreign gene GUS in Arabidopsis thaliana by using Agrobacterium-mediated leaf-infection and detected the expression of GUS gene after ago-inoculation by GUS staining method and semi-quantitative RT-PCR. The results showed that GUS was expressed on 7-15 day after inoculation in Arabidopsis plants. Moreover，we successfully obtained fast expression of the viral suppressor HCPro in Arabidopsis plants, providing a new system for the fast expression of foreign gene and the study of gene function in plants.