H2O2-Scavenging Activity and Hyaluronidase Inhibition Scutellarein and Apigenin in Ocimum basilicum Extract

The potential utilization of flavonoid compounds, scutellarein, and apigenin, contained in Basil leaf extract to manage the aging effects on the skin is not well known. This study was conducted to assess the H 2 O 2 scavenging activity and the inhibition of hyaluronidase from scutellarein and apigenin compounds at various concentrations. The study was conducted by measuring the activity of hydrogen peroxide scavenging and the inhibition of hyaluronidase compounds of scutellarein and apigenin in various concentrations by using spectrophotometry method. The differences in activity were tested using the ANOVA test followed by post hoc Tukey. IC 50 values were calculated based on linear regression equations. The analysis showed the highest H 2 O 2 radical scavenging activity was found in scutellarein compounds with IC 50 at a concentration of 158 μg/mL. Scutellarein compounds tend to have greater scavenging activity than apigenin. Scutellarein was found to have hyaluronidase inhibition with an IC 50 value at a concentration of 35.25 µg/mL, while apigenin was at 162.86 µg/mL. Scutellarein compounds tend to have more inhibitory activity than apigenin. Antioxidant and antiaging effects of basil leaf extract may be caused by phytochemical compounds contained, especially scutellarein.