A method of separating proteins fibrinogen, factor XIII and biological glue a solubilized plasma fraction and preparation of lyophilized concentrates of said proteins

The invention relates to a fibrinogen protein separation process, Factor XIII and biological glue from a solubilized plasma fraction and lyophilized preparation of concentrates of said proteins comprising the steps of: - chromatographic purification comprising the steps of charging an exchanger weak base anion in said solubilized fraction, previously equilibrated by a predetermined ionic strength buffer of basic pH, which allows the retention of the biological glue, elution of the biological glue by increasing the ionic strength of said buffer, and- separation the FXIII from fibrinogen by adding to at least a portion of the biological glue eluate of at least one chemical agent precipitating the FXIII, and recovering the resultant solution of purified fibrinogen supernatant. It also relates to freeze-dried concentrates of said proteins capable of being obtained by the implementation of the process.