Activity of Extracts and Coumarins from the Leaves of Calophyllum brasiliense. on Leishmania braziliensis.

AbstractIn the current study, we evaluated the activity of extracts and (-)mammea A/BB from the leaves of Calophyllum brasiliense. Camb. (Clusiaceae), against Leishmania braziliensis.. The dichloromethane extract and the coumarin (-)mammea A/BB showed significant activity against the promastigote forms of L. braziliensis., with a 50% lethal dose (LD50) at concentrations of 60 and 23.2 μ g/mL, respectively. However, the aqueous extract from the leaves and amentoflavone showed no activity against promastigote forms. For the intracellular amastigote form, the dichloromethane extract and the coumarin (-)mammea A/BB reduced by 50% the infection index of parasites in macrophages at concentrations of 22 and 29 μ g/mL, respectively. In addition, the dichloromethane extract did not show cytotoxic effects against mouse peritoneal macrophages at concentration up to 1000 μ g/mL. On the other hand, the coumarin (-)mammea A/BB showed more cytotoxicity than the dichloromethane extract. The compounds were characterized b...