Characterization of Dissolved Organic Matter and Its Derived Disinfection Byproduct Formation along the Yangtze River

The Yangtze River basin covers one-fifth of China's land area and serves as a water source for one-third of China's population. During long-distance water transport from upstream to downstream, various sources of dissolved organic matter (DOM) lead to considerable variation in DOM properties, significantly impacting water treatability and disinfection byproduct (DBP) formation after chlorination. Using size-exclusion chromatography and fluorescence spectroscopy, the spatial variation in DOM characteristics was comprehensively investigated on a basin scale. The formation of 36 DBPs and speciated total organic halogen in chlorinated samples was determined. Overall, the Yangtze River waters featured a high proportion of terrestrially derived humic substances that served as important precursors for trihalomethanes and haloacetic acids, which was responsible for the increase in total DBP formation along the Yangtze River. The downstream waters were characterized by high levels of microbially derived protein-like biopolymers, which significantly contributed to the formation of haloacetaldehydes and haloacetonitriles that dominated DBP-associated mammalian cell cytotoxicity. Moreover, the precursors of haloacetaldehydes and haloacetonitriles in downstream waters were highly hydrophilic, posing a challenge for water treatment. This study presents an extensive basin-scale study, providing insights into DOM variations along the Yangtze River, illustrating the impact of DOM properties on drinking water from a DBP perspective.