Mutaciones asociadas a resistencia a fármacos antirretrovirales en una población penitenciaria «Naïve»

The objective of this study is to determine the existence of mutations associated with resistance to antiretroviral medication in an HIV positive group of inmates with no previous treatment of this kind. The material and methods used were the following: a descriptive transversal study, which examined HIV genotypes taken from plasmatic RNA samples of HIV positive inmates from Castellon prison. The samples were analysed in the laboratories of the Valencian Institute of Microbiology in Betera (Valencia) by means of PCR double amplification and subsequent sequentiation using a Parker-Elmer automatic sequentiator to determine modes of mutation. The resulting sequences were compared with a wild type of HXB2, which is an HIV prototype. The results were that of the 25 patients selected, one case produced no results due to lack of sufficient genetic material for amplification. The other 24 showed no mutations associated with resistance to N.R.T.I. We found mutations associated with resistance to P.I. in 7 patients (32%), including 4 cases of mutation L63P, 3 cases of mutation V77I and one case with two mutations (L63P and V77I) The results taken from patients with no previous antiretroviral treatment point to the possibility of starting H.A.A.R.T. using empirical methods. However the authors consider that the small size of the sample and the geographical proximity of the patients lead to the need for more studies to confirm the conclusions we have drawn. Key Words: AIDS. Treatment Failure. Prisons