Detection of Carbamate Pesticides based on MWNTs-chitosan Modified Carbon Nanotube Electrode Biosensor with Immobilized Acetylcholinesterase

A stable and highly sensitive enzyme biosensor based on MWNTs-chitosan modified glassy carbon electrode (GCE) with efficient immobilization of acetylcholinesterase (AChE) has been devised for rapid determination of carbamate pesticides especially methomyl, aldicarb, carbaryl and fenobucarb. AChE was immobilized via layer-by-layer self-assembly modification technique. The study indicated that the inhibition of carbamate pesticides on the AChE activity was proportional to concentration of carbamate pesticides in the 10 -10 g·L -1 to 10 -3 g·L -1 concentration range and the limitation of detecting methomyl is up to 10 -12 g·L -1 and others is 10 -11 g∙L -1 . So the enzyme biosensor may be an alternative new tool for analyzing pesticide remains in