Identification of Moghania philippinensis and Moghania macrophylla

Objective: To study the identification methods of Moghania philippinensis and Moghania macrophylla,and to establish a comprehensive precise discrimination method. Methods: TLC and HPLC were applied to analyze genistein in the root of Moghania philippinensis and Moghania macrophylla. DNA barcoding establishment was based on ITS2 sequcence. Results: A comprehensive differentiation method for Moghania philippinensis and Moghania macrophylla based on TLC was proposed,which was combined with HPLC for determination of genistein. The plants of Moghania philippinensis and Moghania macrophylla and their related species could be distinguished by DNA barcoding effectively. Conclusion: TLC and HPLC profiles of Flemingia Radix provide alternative methods of identification using chemical approach. This integrated chemical and molecular approach allows accurate comprehensive fast identification of Moghania philippinensis and Moghania macrophylla,which avoids the methods limitations on the accuracy of identification. The differentiation methods based on TLC,HPLC and DNA barcoding are simple,which provide a new scientific evidence for the identification of authenticity of Flemingia Radix.