Treatment of rat wounds using plasma-jet parameters (Exposure time and gas flow rate)

Worldwide, an estimated millions of patients seek medical care for wounds annually. Different methods and treatment materials have been examined and developed to promote wound healing. Recently, new technology, plasma medicine, has emerged to offer new solutions in wound care. To investigate the efficacy of non-thermal atmospheric pressure plasma jet (NAPPJ) for the treatment of wounds, they were performed 1 cm long and 0.5 cm wide using a surgical scalpel. Treatment was done immediately after the wound operation using an argon plasma jet with different gas flow rates (2.5, 5, 7.5,10 and 12.5slm) and exposure time (3, 6, 9 and 12 min). The treatment was donein a second day. After treatment done the wounds left for one day and in the third day, rat skin tissue has taken and tissue sections had made. The results showed that the mechanical strength of the wound repaired in the plasma treatment group was significantly higher than that in the control group. In addition, evidence from histological studies suggests that wound epithelium is significantly accelerated compared to the control group; vascular and fibrosis (collagen synthesis) increased significantly and the inflammatory stage in wound healing was shorter in the plasma treatment group. The percentage of the necrotic area was higher in the control group than in the plasma treatment group.  Furthermore, exposure to voids within the NAPPJ resulted in a significant reduction in the number of neutrophils infiltrated. NAPPJ exposure to interstitials has a positive effect on wounds that lead to wound healing by limiting the progression of the injury.Also, the results show that the speed of gas flow increasesthe wound healing process andthe speed of gas flow increasesthe active species and accelerates the healing process.