Construction of a microsatellite-based genetic linkage map for Cynoglossus semilaevis

Female and male linkage maps were constructed using SSR markers based on a full sub-family of the tongue sole(Cynoglossus semilaevis).A total of 320 markers were generated in two parents and 92 F1 progenies of the mapping family by using 320 screened SSR primer combinations containing 112 segregation distortion mark-ers(P0.05).The female linkage map included 21 linkage groups and 242 pairs of SSR markers spanning 1311.9 cM with the average marker interval of 4.9 cM and the observed coverage was 83.3%.The male linkage map in-cluded 21 linkage groups and pairs of 218 SSR markers spanning 1 316.2 cM with the average marker interval of 5.5 cM and the observed coverage was 82.0%.The genetic linkage maps are very significant for QTL analyses marker-assisted selection(MAS) breeding programs for economically important traits and sex control in C.semilaevis.