Light-activating chemiluminescence immunoassay kit for serum specific IgE (immunoglobulin E)

The invention provides a light-activating chemiluminescence immunity analysis kit for serum specific IgE (immunoglobulin E). The kit adopts a luminous microsphere coated with a specific single-component allergen (prepared by naturally purification or recombination), a biotin-labeled anti-human-IgE antibody and a light-sensitive microsphere precoated with streptavidin to form detecting reagents. Firstly, to-be-detected serum, the luminous microsphere coated the allergen and the a biotin-labeled anti-human-IgE antibody are incubated together, and the specific IgE antibody in the to-be-detected serum is bound with an antigen molecule on the surface of the luminous microsphere and the biotin-labeled anti-human-IgE antibody respectively; secondly, the light-sensitive microsphere precoated with streptavidin is added for continuous incubation, and is bridged with the luminous microsphere through the binding of biotin and streptavidin; thirdly, a light-activating chemiluminescence analyzing instrument is used for detecting the strength of light signals. The kit has various advantages of being easy and quick to operate, facilitating automation and quantitative analysis, and the like.