Screening and identification of murine MAGE-3 derived,MHC class II restricted peptide epitope

To screen murine MAGE,3 derived, MHC class II restricted peptide epitope, we used a MHC class II epitope prediction algorithm to select five candicate peptides according to the score. The immunogenicity and immune reactivity of peptide were evaluated on the basis of T,cell proliferation assay, specific recognition of tumor cells and cytokines release. The experimental results showed that DC loaded with MAGE,322,36 elicited potent T cells proliferation, and MAGE,322,36 pulsed DC activated T cells specificially recognized MAGE,3 expressing tumor cells in MHC class II restricted manner. MAGE,322,36 pulsed DC induced T cells population was mainly CD4+ Th1 cells, and NK, NKT, γ/δT in the innate immune system were activated concomitantly. It concludes that MAGE,322,36 was identified as a MHC class II restricted epitope.