Construction and function analysis of growth hormone secretagogue receptor promoter

Objective To analyze the structure and function of growth hormone secretagogue receptor (GHS-R) promoter region primarily by using the constructed recombinant reporter gene promoter pGB-enhancer vectors containing different lengths of GHS-R promoter deletion mutants. Methods The recombinant plasmids were transfected into CH3 cells, then the promoter activities of different recombinant plasmids were detected by the Dual Luciferase Reporter Assay System. Results It was found that the promot er activities of pGL3-Enhancer-A,-B,-C,-D, and-E were (2. 30 ± 0. 21), (8.40 ±0.86) , (5.20 ± 0.30), (10.60 ±0.54) and (14.10 ±0.74) respectively,and there was significant difference between pGL3-Enhancer A,and-B,pGL3-Enhancer-B and-C, between pGL3-Enhancer-C, and-D,and between pGL3-Enhancer-D and-E (all P<0.05). Conclusion There was a positive transcriptional regulation region from-254 to-168,-625 to-355,-910 to-625 respectively,and a negative transcriptional regulation region be tween-355 to-254 in the upstream of the human GHS-R promoter. Key words: GHS-R gene;  Promoter;  Mutants;  Transfection