A survey of Sam Orr's Pond (New Brunswick, Canada) uncovers the invasive green alga Codium fragile (Chlorophyta) and the orange-striped green anemone Diadumene lineata (Cnidaria), first records for the Bay of Fundy and Canada, respectively

A DNA-barcode-based bio-inventory of macroalgae and macro-invertebrates in Sam Orr’s Pond, New Brunswick, was completed as part of the Canadian Barcode of Life Network’s efforts to generate a comprehensive comparative DNA database (BOLD) for rapid species identification. During an early sampling episode the invasive species Codium fragile was identified and subsequently confirmed with tufA sequence data – the first record of this species in the Bay of Fundy. An unidentified sea anemone was also collected, but COI-5P data failed to find a match in current databases (BOLD and GenBank). Subsequent morphological identification resulted in the first Canadian records for Diadumene lineata. Our discoveries highlight the importance of ongoing field monitoring, trained taxonomic expertise and comprehensive DNA barcode libraries in global efforts to track the spread of introduced species.