A method for extracting complex enzyme cantharidin

The invention relates to a method for extracting cantharidin from compound enzyme. The method comprises the following steps of: performing enzymolysis on a mylabris medicinal material with cellulase and pectic enzyme; leaching a hydrolysate with a mixed liquid with the volume ratio of chloroform to absolute ethanol to concentrated hydrochloric acid of (1-2):(1-2):(1-2); performing suction filtration on a leached product in hot state and adding chloroform-absolute ethanol-concentrated hydrochloric acid in the volume ratio of (1-2):(1-2):(1-2) for leaching; combining filtrates obtained in two times and performing rotary steaming; dissolving the obtained solid by using a mixed liquid in the volume ratio of water to chloroform of (1-2):(1-2); extracting a lower layer organic phase and performing rotary steaming on the lower layer organic phase; adding a mixed liquor in the volume ratio of absolute ethanol to petroleum ether of (1-2):(1-2) into the obtained solid for dissolving; and recrystallizing with acetone to obtain cantharidin. Compared with the conventional extraction method, the method has the advantages of high extracting speed, low cost, time saving, labor saving, high extraction rate, lower impurity content, easiness for purification and the like, and is suitable for large-scale industrial production.