Quantification of Sugars and Organic Acids in Biological Matrices Using GC-QqQ-MS
2018
Gas chromatography coupled with triple quadrupole mass spectrometry (GC-QqQ-MS) can be used to
accurately quantify endogenous small molecules extracted from biological samples such as plants and
human fluids including sera and urine. In order to quantify primary metabolites typically from central
carbon metabolism such as sugars from glycolysis and the pentose phosphate pathway; and organic acids
involved in the tricarboxylic acid (TCA) cycle; polar endogenous metabolites must be extracted from the
samples of interest, chemically derivatized and quantified against a linear calibration curve to a
corresponding authentic standard. This chapter describes how to quantify a combination of 48 primary
metabolites belonging to classes of sugars, sugar alcohols, sugar acids, sugar phosphates, and organic acids
using a robust, optimized, multiple reaction monitoring (MRM)-based GC-QqQ-MS method.
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