Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco

Siham Fellahi,Mehdi El Harrak,Jens H. Kuhn,Ghizlane Sebbar,E. Bouaiti,Khadija Khataby,Ouafae Fassi Fihri,Mohammed El-Houadfi,My Mustapha Ennaji

Comparison of SYBR green I real-time RT-PCR with conventional agarose gel-based RT-PCR for the diagnosis of infectious bronchitis virus infection in chickens in Morocco

2016

Siham Fellahi
Mehdi El Harrak
Jens H. Kuhn
Ghizlane Sebbar
E. Bouaiti
Khadija Khataby
Ouafae Fassi Fihri
Mohammed El-Houadfi
My Mustapha Ennaji

Background A rapid, sensitive, and specific molecular method for the diagnosis of infectious bronchitis virus (IBV) infection is important in curbing infectious bronchitis outbreaks in Morocco and other countries.

Keywords:

Bronchitis
Coronavirus
Virus
Microbiology
Agarose
Gammacoronavirus
Outbreak
SYBR Green I
Virology
Medicine
Real-time polymerase chain reaction
infectious bronchitis virus
gel based

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