Effect of Graphene on Nonneuronal and Neuronal Cell Viability and Stress

In recent years graphene has drawn considerable research interest for biomedical applications. However, applications of graphene in biological systems also raise concerns about its possible toxicity. Here, by using live cell imaging techniques, we investigate the effect of pristine graphene on the viability as well as stress of both nonneuronal and neuronal cells under physiological conditions. We find that graphene promotes cell adhesion and proliferation. Furthermore, we find that graphene has no detectable adverse effect on mitochondrial membrane potential and morphology, or autophagy levels in the cell, indicating that graphene does not induce cell stress. Our results highlight the potential of graphene to be used in biomedical applications by providing long-term and stable nonneural and neural interfaces.