Study of Activity of Schwann Cells Cultured in Threes Kind of Scaffold of Artificial Biodegradable in Vitro

Objective To compare the activity of Schwann cells cultured in different scaffold of artificial degradation in vitro.Methods To choose bFGF Chitosan-collagen Sponge,absorbable gelatin sponge and PLGA degradable supporting materials.Primary cultured Schwann cells were used to seed on supporting materials.Then,the Schwann cells of rat were isolated and cultured by sequential enzyme digestion.MTT assay was employed to observe the proliferation of Schwann cells,Determination of cell cycle and apoptosis by flowcytometer,shape of cells and structure of materials observed by environment scanning electron microscope,to judge the activity of Schwann cells.Results The Schwann cells may be cultured on bFGF Chitosan-collagen sponge,absorbable gelatin sponge and PLGA,but best for bFGF Chitosan-collagen Sponge.Conclusion The bFGF Chitosan-collagen Sponge seeded with Schwann cells were suitable to construct tissue-engineered bioartificial nerve.