Cloning and expression characterization of the full length cDNA for a novel leukemia-associated gene LRP16

Firstly, the human ESTs (Expression Sequence Tags) fragments obtained from electronic hybridization were assembled using a 3 kb DNA fragment cloned as probe, then designed the primers for rapid amplification of cDNA end (RACE). Tissue expression pattern were analysed by Northern blotting, and bioinformatic data of High Throughout Genomic Sequences (HTGS) were used for chromosome localization. Prokaryotic recombination protein was induced by using IPTG and bacterial protein electrophoresed on SDS-PAGE. The inserted fragments of recombinant were confirmed by sequencing. The full length cDNA of the gene LRP16 was cloned and its translated amino acid sequence was deduced. This gene was localized on chromosome 11q12.2. One recombinant whose sequence had 30 bases deleted was obtained. LRP16 gene might produce two types of proteins and the only difference of the two was the length of their N-terminus. This gene might have two types of splicing transcripts and the shorter lower transcripting.