189 Molecular and pharmacological characterization of primary mesothelioma tumor cell lines orthotopically xenografted in nude mice

library were cultured for 13 days, after which shRNAs were recovered by PCR. Deep sequencing was applied to determine the relative abundance of each shRNA in BRAF (V600E) cells as compared to WT2 CC cells. Results and Conclusions: Based on the results of the pooled shRNA screen, we were able to identify six candidate synthetic lethal genes in BRAF mutant CC cell lines. In particular, further validation showed RANBP2 gene knock-down to be synthetic lethal with BRAFV600E and BRAFlikeness in CC. Experiments addressing the identification and the biological characterization of RANBP2 will be presented.