[Study on the epidemiological of rotavirus diarrhea in Lulong in 2008-2009]

OBJECTIVE: To analyze the feature of epidemiological of rotavirus diarrhea in Lulong county Hebei province. METHODS: 426 stool specimens were collected from inpatant with acute diarrhea from children less than 5 years old. Rotavirus-positive specimens were identified by ELISA kit. G/P typing assays were confirmed with multiplex seminested RT-PCR. RESULTS: Rotavirus was detected in 202 of 426 (47.42%) specimens. Genotyping of rotavirus showed that G3 was predominant (57.9%) followed by Gmix (16.3%) G9 (14.9% ) G1 (7.9%) G4 (1%) G2 (0.5%) P-genotyping showed that P [8] Pmix P [4] P [9] type were found in 58.4% 28.7% 6.9% and 1% respectively. The most common G/P combination identified was G3P [8]. CONCLUSION: Group A rotaviruses was a major pathogen of diarrhea in Children in Lulong. G3P [8] was the predominant type in 2009 Gmix and Pmix abound and G9 serotypes has become the second predominant after G3 strain in the region.