Overexpression of the SARS-CoV-2 receptor ACE2 is induced by cigarette smoke in bronchial and alveolar epithelia

Abstract Angiotensin-converting enzyme 2 (ACE2) has been identified as the functional receptor of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and a target for disease prevention However, the relationship between ACE2 expression and its clinical implications in SARS-CoV-2 pathogenesis remain unknown Here, we explored the location and expression of ACE2, and its correlation with gender, age and cigarette smoke (CS), in a CS-exposed mouse model and 224 non-malignant lung tissues (125 non-smokers, 81 current smokers and 18 ex-smokers) by immunohistochemistry Moreover, the correlations of ACE2 with CS-induced oxidative stress-related markers, hypoxia inducible factor-1α (HIF-1α), inducible nitric oxide synthase (iNOS), and 4-hydroxynonenal (4-HNE) were investigated Chromatin immunoprecipitation and luciferase reporter assays identified the cause of ACE2 overexpression in human primary lung epithelial cells We demonstrated that ACE2 was predominantly overexpressed on the apical surface of bronchial epithelium, while reduced in alveolar epithelium, owing to the dramatically decreased abundance of alveolar type II pneumocytes in CS-exposed mouse lungs Consistent with this, ACE2 was primarily significantly overexpressed in human bronchial and alveolar epithelial cells in smokers regardless of age or gender Decreased ACE2 expression was observed in bronchial epithelial cells from ex-smokers compared with current-smokers, especially in those who had ceased smoking for more than 10?years Moreover, ACE2 expression was positively correlated with the levels of HIF-1α, iNOS, and 4-HNE in both mouse and human bronchioles The results were further validated using a public available dataset from The Cancer Genome Atlas (TCGA) and our previous integrated data from Affymetrix U133 Plus2 0 microarray (AE-meta) Finally, our results showed that HIF-1α transcriptionally upregulates ACE2 expression Our results indicate that smoking-induced ACE2 overexpression in the apical surface of bronchial epithelial cells provide a route by which SARS-CoV-2 enters host cells, which supports clinical relevance in attenuating the potential transmission risk of COVID-19 in smoking populations by smoking cessation This article is protected by copyright All rights reserved