Effect of JKA97 in inducement of apoptosis of HepG2 cells and its molecular mechanism.

Objective:To investigate the inhibitory effect of JKA97 on proliferation and the molecular mechanism of human liver cancer cell line HepG2 in vitro.Methods:The cell viability was detected by MTT[3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrasoliumBromide];Morphological changes of HepG2 cells were measured by microscope;JKA97 induced apoptosis of HepG2 cells was detected by AnnexinV-PI staining and flow cytometry;The expression of mitochondria-shaping protein,mitofusin-2(mfn2)was measured by Western blot.Results:JKA97 induced the apoptosis of HepG2 cells and the expression level of mfn2 decreaed gradually. Conclusion:JKA97 can obviously inhibit the proliferation of human liver cancer cell line HepG2 in vitro and induce apoptosis of HepG2 cells.Lower expression of mitochondrial fusion protein mfn2 may be one of JKA97's molecular mechanisms.